Feedstuffs Analysis - Crude Fat (Hexane Extractables)

Subject: Crude Fat (Hexane Extractables)

Introduction

Grind a 50-100 g sample to a uniform particle size (10 mesh) in a grinder or cutting mill (Note 3). Mix the sample thoroughly and weigh accurately about 4 to 5 g into the steel grinding vial. Add one 12 mm and two 6 mm steel balls and 25 mL of n-hexane. Seal the vial and mill in the Mixer/Mill for 15 mins.

Place a filter paper in the funnel and filter the milled sample slurry into a clean and dry vacuum flask (Note 4). Quantitatively transfer the residual oil from the steel milling vial and cap with the aid of three 10 mL portions of hexane, and wash residual oil from the sample with another three 10 mL portions of hexane. Transfer the filtered hexane and oil miscella into a dried and tared 150 mL beaker. Rinse the vacuum flask residue into the beaker with small portions of hexane. Evaporate the solvent on a steam heated water bath under the hood (Note 5). Place the beaker with residue in the air oven and dry it 1 hour at 100 °C (Note 6). Remove the beaker and cool in a desiccator. Weigh the beaker and calculate the dry extract weight.

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